Composite

Part:BBa_K3440006:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


RhlI-Myc under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 722
    Illegal BglII site found at 760
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to show that RhlI can be expressed under constitutive promoter. A myc tag was added for characterization. A moderate promoter (BBa_B0034) was used.

Source

RhlI from Pseudomonas aeruginosa

References